Synthetic DNA Molecules with Catalytic and Binding Properties as Sensors
报告内容：Our laboratory has been interested in developing simple biosensing devices or assays that use two classesof synthetic DNA molecules: DNA aptamers (DNA-based molecular receptors) and DNAzymes(catalytic DNAs). For example, we have successfully developed DNAzymes that are capable of trackingEscherichia coli (a common water/food-borne pathogen) and Clostridium difficile (a common causefor healthcare- associated infections in North America and Europe), and currently we are working ondeveloping similar DNAzymes for other important human pathogens. Furthermore, given the fact thatDNA is a highly programmable material based on predictable WatsonCrick base- pairing interactions, wehave been interested in engineering molecular devices in which a key player is a DNA aptamer or ligand-responsive DNAzyme to achieve ultra-sensitive target detection. For example, we have developed severalsimple biosensing DNA devices/assays built with various linear and circular aptamer/ DNAzyme-containingcomplexes that can be activated by a target of interest. In this presentation, I will discuss the progress we have made and challenges we have encountered from these efforts. 报告人简介：Yingfu Li was born and raised in Anhui, China. He received his BSc in chemistry at Anhui University in1983, and his MSc in applied chemistry at Beijing Agriculture University in 1989. He moved to Canada in1992, and in 1997, he graduated with a PhD in chemistry and biochemistry at Simon Fraser Universityunder the supervision of Professor Dipankar Sen. His doctoral study led to the discovery of one of theearliest DNA molecules with catalytic ability using a powerful test-tube evolution technique knownas invitro selection. This work won him Governor General of Canada Gold Medal (1998) and a Doctoral Prizefrom the Natural Sciences and Engineering Research Council of Canada (1998). He received a Medical Research Council of Canada postdoctoral fellow in 1997 and spent the next two years in the laboratory ofRonald Breaker at Yale University to study artificial evolution using DNA as the model system. Throughtest-tube evolution he derived a series of nucleic acid enzymes that are capable of modifying DNA usingchemical reactions that are carried out in cells by protein enzymes. In 1999, he joined McMaster University as an assistant professor in the Department of Biochemistry and Biomedical Sciences and theDepartment of Chemistry and Chemical Biology, was promoted to the rank of associate professor in 2005 and full professor in 2O10. At McMaster, he has established a research group to take advantage of thepowerful test-tube evolution technique to develop artificial DNA and RNA molecules that can be used toaddress fundamental biochemical and evolutionary questions or can be utilized for wide-rangingapplications. He has published extensively in the fields of chemistry, biochemistry and molecular evolution of nucleic acids. He has published ~18O research and review articles, ~ 20 book chapters, edited1 book, and filed over 20 patents. He also serves as an associate editor of Journal of Molecular Evolutionand as a member of editorial board of Scientific Reports. He has received several recognitions, includingCanada Research Chair, New Investigator Award from the Canadian Institute of Health Research (CIHR),Premier Research Excellent Award from Ontario Government, and W. A. McBryde Medal from CanadianSociety of Chemistry.